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Similar content being viewed by others 1 Introduction Pregnant women have an increased susceptibility to depression during and shortly after pregnancy (Gelaye et al., 2016)

These conditions often involve chronic low-grade inflammation, cartilage degradation, and synovial fluid changes

Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

Eventually, the sequential activation of caspase 3 (CASP3), a principal executor of apoptosis, is induced by the activation of initiator CASP8 or CASP10 via cell death receptor pathways, and CASP9 via mitochondrial pathways