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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

Furthermore, 58 M Cu(OAc) 2 also upregulated the IL-1 expression significantly

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NAD+ 1000 mg Lyophilized Powder + 10 ml Bacteriostatic Water (2-Vial Research Kit) 1000 mg + 10 ml Sterile research-use set with one vial of lyophilized NAD+ and one vial of bacteriostatic water for reconstitution