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[DOI] [PubMed] [Google Scholar] 54.Moon KH, Hood BL, Mukhopadhyay P, Rajesh M, Abdelmegeed MA, Kwon YI, Conrads TP, Veenstra TD, Song BJ, Pacher P

A Randomized Double-blind Placebo Controlled Phase 3 Trial to Evaluate the Efficacy and Safety of Estetrol for the Treatment of Moderate to Severe Vasomotor Symptoms in Postmenopausal Women (E4Comfort Study I)

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A prepacked Ni-NTA column was equilibrated with dialysis buffer and the flowthrough was collected and concentrated to 2 mL using a 10 kDa MWCO concentrator (Amicon Ultra 15, Millipore)