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Acknowledgements This investigation was funded in part by the National Institutes of Health through grants R01 NS055095 (RAG) and in a facility constructed with support from Research Facilities Improvement Program Grant C06 RR15482 from the National Center for Research Resources, NIH

A successful needle insertion is indicated by a clear sense of penetration and a somewhat resistant retraction, with only air being aspirated back into the syringe

Recently, Harada et al

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