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SNAP/FLAG-hGLP-1R serine phosphorylation analysis by immunoprecipitation INS-1 832/3 GLP-1R KO cells were seeded in 6-well plates 24 hours before transfection with 2 g WT or phospho-site mutant SNAP/FLAG-hGLP-1R constructs using Lipofectamine 2000 (Thermo Fisher Scientific) as per the manufacturers instructions, with all subsequent transfections in the study also performed with this reagent