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Simultaneous mutation of all three guiding bar residues (Trp407 hTrxR1 , Asn418 hTrxR1 , and Asn419 hTrxR1 ) only led to a minor increase in catalytic efficiency of hTrxR1 compared to the Asn419 hTrxR1 mutation (Table 2)
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Although there are multiple of other targets such as inactivation of sodium channel [139] and enhancement of -aminobutyric acid (GABA)-mediated inhibitory neurotransmission [140], pan HDACs inhibition is believed to play a critical role for the therapeutic value of VPA as mentioned above
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