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If the process feels opaque or clunky, assume other core functions may be similarly neglected
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WB showed increased p-mTOR, Raptor, p-4E-BP1, p-S6, Bcl-2 and p62, while decreased Bax, Apaf-1, cleaved caspase-3, Beclin, and LC3-I/II
For most researchers, 2.5 mg/mL represents the best balance between accuracy and convenience
