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Calcium imaging Co-cultures of ND neurons and GLUTag cells were loaded with 5 M Fura-2-acetoxymethyl ester (Molecular Probes) for 30 min in standard saline and Fura-2 calcium imaging was performed with an inverted fluorescence microscope (Olympus IX71, UK) with a 40 oil-immersion objective, coupled to a 75 W xenon arc lamp and a monochromator (Cairn Research, Faversham, UK) controlled by MetaFluor software (Molecular Devices, UK)