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For recruitment assays, INS-1 832/3 GLP-1R KO cells were seeded onto 6-well plates and transiently transfected with a combination of 1 g GLP-1R-SmBiT WT or V229A and either 1 g of LgBiT-mini-G s , -mini-G q or --arrestin 2

This method attempts to normalize dosing across body sizes but lacks clinical validation

doi: 10.3390/antiox13010034 132 MengXLiYLiSZhouYGanRYXuDPet al

Retatrutide is a novel once-weekly triple agonist for the GLP-1R, GIPR, and GCGR receptors, showing a stronger action on the human GIP receptor, and exerting weaker effects on the GLP-1 and glucagon receptors