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When the fluorescent secondary antibody method was used, the sections were treated with Alexa 488- or Alexa 555-conjugated anti-mouse/rabbit IgG (1:500 dilution, Molecular Probes, Eugene, OR)

Throughout these evaluations, GHK appeared to lower ROS levels induced by tert-butyl hydroperoxide (t-BOPD), a chemical known to promote oxidative stress within cells