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GSH level, SOD and GST activities were significantly decreased, and NO level and TF activity did not significantly change in the MTX group compared with the control group

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After incubation with the appropriate secondary antibodies (Peroxidase AffiniPure Goat Anti-Mouse, 115-035-146, or Peroxidase AffiniPure Goat Anti-Rabbit, 115-035-144, both from Jackson Laboratories), membranes were immersed in enhanced chemiluminescent (ECL) substrate solution (#34080