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The number of living cells were counted by using an automated cell counter (Countess II, Thermo Fisher Scientific Inc.) after staining with trypan blue by pipetting for around 10 s

For the cerebrovascular uptake study ( n = 22), male and female homozygous APOE2-, APOE3-, and APOE4-TR mice were euthanized at 26-weeks (Supplementary Table S2)

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Left out (powder) Left out (reconstituted) Arrived warm (powder) Arrived warm (reconstituted) Power cut (powder) Power cut (reconstituted) Freeze-thaw concern (powder) Freeze-thaw concern (reconstituted) Traveling with it (powder) Traveling with it (reconstituted) Basis (why this answer is reliable) Confidence This scenario is often not a write-off if corrected promptly