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Khavinson and his collaborators have published the bulk of the existing research on it across roughly 25-30 years of work ranging from cell culture experiments through animal lifespan studies into human clinical observations

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While the IOM had based B12 needs on the amount required to correct hematological parameters for people with pernicious anemia, the EFSA had almost 20 more years of research to draw on, and concluded that the most suitable approach was basing needs on biomarkers (serum B12, holotranscobalamin, MMA, and homocysteine)