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For the survival experiment, mice were checked daily and were culled when they showed visible distress symptoms such as weight loss of 10% or more, ascites equivalent to full-term pregnancy, reduced or slow activity, pale feet, hunching, piloerection, closed eyes and isolation from cage mates

10.1523/jneurosci.17-08-02921.1997 J

Three aliquots from each subject were analyzed by NADMED method and another three by targeted LC-MS method

June 29-30, 2009: Joint meeting of the Drug Safety and Risk Management Advisory Committee with the Anesthetic and Life Support Drugs Advisory Committee and the Nonprescription Drugs Advisory Committee: FDA briefing material