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Supernatant was collected by centrifugation at 30,000 rpm for 30 min

Insulin Feedback: The insulin secreted by -cells acts as a negative feedback signal to further inhibit glucagon release from -cells (Zheng et al., 2024)

As a GLP-1 receptor agonist, liraglutide works by enhancing glucose-dependent insulin secretion, suppressing glucagon release, slowing gastric emptying, and promoting satiety