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Quiescent in-transit melanoma cells are resistant to therapies, suggesting that these melanoma cells may have transdifferentiated into endothelial-like cells and contribute to melanoma relapse in patients who have previously responded to therapy (73)

Proteins from each treatment were loaded in technical duplicate, resolved by SDS-PAGE on NuPAGE 4 to 12%, Bis-Tris, 1.0 mm polyacrylamide gels (Invitrogen) and transferred to a PVDF membrane using the iBlot 2 Transfer Stacks, PVDF, mini and Gel Transfer Device (Invitrogen)

However, the best peptide depends on the type of inflammation being addressed