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By advancing the accuracy and scalability of in vitro liver models, this work contributes to reducing reliance on animal testing and promoting ethical innovation in preclinical research, as emphasized by Ingelman-Sundberg and Lauschke 43

Curcumin induces crosstalk between autophagy and apoptosis mediated by calcium release from the endoplasmic reticulum, lysosomal destabilization and mitochondrial events

Accumulation of electrophilic aldehydes during postovulatory aging of mouse oocytes causes reduced fertility, oxidative stress, and apoptosis

The abdominal fat (stomach) is more commonly used due to its ease of access and large surface area, but the thigh is a perfectly viable alternative if preferred