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Argyridou S, Bernieh D, Henson J, Edwardson CL, Davies MJ, et al

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H3-D1R heteromers also couple to Gi to direct histaminergic input towards -arrestin/MAPK pathway within the GABAergic neurons (64) with an increased phosphorylation of rpS6 and transient phosphorylation of GSK3 (65), and affect both rapid receptors signaling like Ca2+ mobilization and longer cell signaling pathways like p38, involving neuronal cell death (66)

This basal medium was then augmented with a specific cocktail of small molecules: 3 M or 1 M CHIR99021 (CHIR), IDE1, 2 M Ly294002 (Ly), and 0.75 M PD0332991 (PD)