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Trial #4 Compared tirzepatide with people on metformin, and/or sulfonylureas and/or SGLT-2s with insulin Glargine on individuals with high cardiovascular (heart and blood vessels disease) risk
Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18
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