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MTT assay NIH/3T3 cells were seeded on 96-well plates (5 10 3 cells per well) and cultured for 24 h

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Proactive Fluid Targets Set a baseline daily fluid goal of 2-3 liters of water and electrolyte-containing beverages before GI symptoms develop

Clinically, this response would result in the development of inflammatory nodules and the formation of abscesses and draining tunnels or fistulas.23,25 Specifically, the activation of macrophages, the inflammasome and dendritic cells would cause the release of various pro-inflammatory cytokines such as TNF-, IL-1, IL-6, IL-8, IL-12 and IL-23.25 Dendritic cells, through the production of IL-12 and IL-23, would promote the activation of the adaptive immune system, with the expansion and differentiation of Th1 and Th17 cells.23,26 Th1 cells produce interferon gamma (IFN- ) and Th17 cells produce IL-17, IL-22 and TNF-, amplifying the inflammatory cascade