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After removing duplicates and screening publications according to inclusion criteria (for a flow diagram, see Supplementary Figure 1), five articles remained (Table 1)

Monitoring the effluent at 220 nm, chromatography (flow rate: 1.0 mL/min) was started with a mixture of H 2 O/MeOH (40/60, v/v), the MeOH content was kept constant for 1 min at 60%, increased within 20 min to 80%, increased within 5 min to 86%, increased within 10 min to 90%, increased within 1 min to 100%, kept constant for 3 min, decreased within 1 min to 60% MeOH, followed by re-equilibration for 4 min

2007, 7: 101- 10.1186/1471-2180-7-101 Acknowledgments The authors thankfully acknowledge the support from Indian Council of Agricultural Research (ICAR), New Delhi and The Director, National Dairy Research Institute (NDRI) Karnal, India

A- According to Trade India, the price of this injection is 65.00 INR / vial.