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Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

Individual variation is substantial, making personalized assessment valuable

Improvements in sleep quality, particularly if breathing during sleep improves, may make dreams feel more noticeable." On when to seek help: "If dreams become distressing, lead to sleep avoidance, or occur alongside symptoms such as nighttime confusion or palpitations, it is sensible to speak with a GP