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A 23-G needle was used to slowly inject 0.5 ml cold PBS with 0.1 mM EDTA into the left lung, and then the BAL fluid was collected from the lungs

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Published preclinical and in vitro research has examined its interaction with extracellular matrix remodelling, broad gene-expression modulation, antioxidant and anti-inflammatory pathways, and copper-coordination chemistry in cultured fibroblasts and animal wound models

PIO was the first antihyperglycemic medication that demonstrated beneficial liver effects in a small RCT, which analyzed data from 55 individuals with prediabetes/diabetes and biopsy-proven NASH [48]