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DOI:10.1016/j.burns.2004.10.013 6.Srensen.E, Cowland.J, Ganz.T, Borregaard.N, Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors

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Immunoblotting The dishes were washed 2 with ice-cold PBS, lysed in 1 Laemli sample buffer, and subjected to standard SDS-PAGE using separate 4%12% Bis-Tris gels, after which the proteins were overnight transferred at 4C to polyvinylidene fluoride membranes