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After initial growth to an OD600 of 0.8, cells were induced using 0.4 mM isopropyl -D-1-thiogalactopyranoside (IPTG) and cultured at 20C for 16 hours

Gastroenterology 167, 689703

PubMed Evidence that BMR decreases proportionally with sustained weight loss supporting the need to recalculate TDEE at intervals during GLP-1 treatment.