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Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81

After the addition of chloramine-T to oxidize the hydroxyproline residues, samples were supplemented with Ehrlichs aldehyde reagent and incubated at 65 C for chromophore development

Department of Agriculture (USDA), 100 grams of raw, seedless watermelon contains: Its also rich in nutrients, including: Watermelon rinds have less sugar and more fiber than their flesh does