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For co-IP, proteins from primary astrocytes were harvested by lysis in cell lysis buffer (150 mM NaCl, 0.5% NP-40, 10% glycerol, 2 mM mercaptoethanol, 0.2 mM phenylmethylsulfonyl fluoride, and 10 mM glycerophosphate) after experimental treatments

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CNS spectrums , 27 (3), 281289