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Injecting slowly, over 20 to 30 seconds rather than in a single rapid push, allows the surrounding subcutaneous tissue more time to equilibrate as the solution is introduced
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In the following year, Svante August Arrhenius (18591927) published a paper on the viscosity of colloidal solutions (including proteins), where he used physical chemistry principles to explain how the concentration and denaturation of proteins affect the viscosity of biological fluids (Kunitz, 1926)
It goes right through the nasal mucosa into the blood
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