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DOSY spectra were measured using the stimulated echo sequence with a longitudinal-eddy-current delay 62,63 , and diffusion coefficients were calculated from signals in the aliphatic regions using the software TOPSPIN (Bruker)

This is critical for longitudinal research and multi-institutional collaborations that require standardized materials

The accumulation of ROS leads to the opening of the mitochondrial permeability transition pore (mPTP) and dissipation of the mitochondrial membrane potential, resulting in a surge of ROS in a phenomenon termed ROS-induced ROS release (RIRR) [46]

Individual outcomes depend on lifestyle adherence, metabolic factors, genetic predispositions, and treatment duration