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Then, total RNAs weighing 2 g were used for the library preparation of stranded RNA sequencing using the KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina (catalog number DR08502, Wuhan Seqhealth Co., Ltd., China) based on the manufacturers instruction

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Metabolic and Molecular Effects of Testosterone in Skeletal Muscle Anabolic effects of AR and testosterone upregulation after RE occur through a combination of both genomic i.e., transcriptional capacity, and non-genomic i.e., translational efficiency, pathways (Kraemer et al., 2020)