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Prior to the assay, cells were washed and incubated at 37C in a non-CO 2 incubator for equilibration

For decades, GPX typically uses glutathione (GSH) as a reducing agent in catalyzing the reduction of H 2 O 2 or organic hydrogen peroxide to water or corresponding alcohols, respectively ( 2 O 2 and rapid reduction by GSH (Zhao et al., 2019)

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During chronic inflammation and oxidative stress, astrocytes release toxic inflammatory mediators and free radicals, accelerating activation of microglia and neurodegeneration [65]