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10 M retinoic acid was used as a positive control for maturation towards a differentiated neuronal phenotype, as shown previously ( 2.2 DPI synergizes with MitoQ in MNA NB cells To assess whether MitoQ and DPI act synergistically in reducing cell viability, we treated two MNA NB cell lines (IMR-5/75 and Be(2)-C) with various concentrations of DPI and MitoQ

This inhibition reduces intracellular 1-methylnicotinamide (1-MNA) production (EC50 = 2.3 M) and preserves nicotinamide availability for the NAD+ salvage pathway

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Recent protocols suggest methylcobalamin may offer better neurological outcomes