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Symptoms of DPN vary according to the class of involved sensory fibers, presenting with pain and unpleasant sensations if small fibers are involved and with numbness and loss of protective sensation (LOPS) if large fibers are involved

the freeze-drying process comprises the following steps, preparing a liquid and filling at the temperature of 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 2-4 h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 for 2-4 h, then heating the plate layer to 10 within 3h, when a product is heated to 0 , starting to increase the temperature of the plate layer to 40 , and preserving heat for 4-6 h after the product is heated to 30

doi: 10.1126/scisignal.abm6265 120 YaoFDengYZhaoYMeiYZhangYLiuXet al