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If you do not notice meaningful benefit after an appropriate number of sessions, we will discuss alternatives rather than continuing indefinitely without evidence of benefit

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After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)