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It is constrained by the natural variation of the desired trait present in the available collection of crop germplasm, as well as by being time-consuming

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These procedures may identify individuals who can avoid regional lymph node dissection and individuals who may benefit from adjuvant therapy.[5-10] Multiple studies have demonstrated the diagnostic accuracy of SLNB, with false-negative rates of 0% to 2%.[5,10-15] To ensure accurate identification of the sentinel lymph node, lymphatic mapping and removal of the sentinel lymph node are ideally performed during the same operation as the wide excision of the primary melanoma