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Additionally, the interaction between follicular helper T cells and B cells plays a crucial role in the formation of tertiary lymphoid structures, which promote local antibody responses at sites of chronic inflammation [52]

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The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4C overnight, then the blots were rinsed 35 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature

Advanced analytical procedures including LCMS screening, heavy metal analysis, endotoxin testing, and sterility verification are routinely performed across commonly studied compounds