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The data suggested sustained improvements at all maintenance levels, though again the greatest benefits aligned with the highest dosage

Side Effects : GLP-1 often causes nausea and headaches, while Metformin leads to diarrhea and bloating

While it has been studied over one to two years in clinical trials, there is limited data beyond that time frame, especially regarding: Long-term metabolic effects Nutrient deficiencies due to reduced food intake Potential changes to gut microbiota Dependency on the drug to maintain weight loss Potential changes to gut microbiota Some clinicians have voiced concern that stopping Ozempic can result in rapid weight regain and the return of previous metabolic issues

RT-qPCR procedure was performed as mentioned before with the following primers (Table 2)