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Costanzo G, Curatolo S, Bus B, et al

Cells were then labelled using SNAP-Surface-549 (1 M) in complete medium for 30 minutes on ice to label surface receptor (contributed to by a recycled receptor population and a further population that were not internalised during the agonist stimulation step), fixed with 4% paraformaldehyde, and mounted in Diamond Prolong mounting medium