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For colony formation assays, cells (5 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar

Collecting duct water permeability inhibition by EGF is associated with decreased cAMP, PKA activity, and AQP2 phosphorylation at Ser(269)

doi: 10.1186/s13046-024-02994-0 77 PengchengZQuanchengWZhibinLPeijunYKefengDRuohanZ