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10.1523/JNEUROSCI.2084-10.2010 J Neurosci

The following day the medium was discarded, cells washed with HBSS and loaded with SSip-1 DA in DMEM at a concentration of 5 M for 1 h at 37C

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From its origins in structure-guided drug design to its current applications across multiple research disciplines, 5-amino-1MQ exemplifies how targeted enzyme inhibition can provide insights into complex biological systems while maintaining the specificity required for rigorous scientific investigation