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2.4 Safflower aqueous extract preparation For daily treatment, 5 g of the powdered flowers are macerated in 100 mL of heated distilled water for 1 h until they cool, and then used ( 2.5 HPLC analysis of safflower aqueous extract The polyphenols of safflower aqueous extract were determined using the HPLC system at the National Research Center (NRC) in Giza, Egypt, according to UV and diode array detection (DAD) methods ( 2.6 Collection of blood and samples After the experiment period (4 weeks) and following a 12-h fast, the rats were euthanized by CO 2 ( 2 flow by displacing 30%70% of the cage volume per minute until narcosis without removing them from their home to avoid stress caused by handling and then the narcotic rats were subjected to cervical dislocation

Cell 2008;135:3915

After just a few nights, we already started to see big sleep improvements, and we worked confidently towards reducing our sons night wakings and the duration of his night feeds

doi: 10.3389/fphar.2025.1689522 Received 28 August 2025 Accepted 08 October 2025 Published 23 October 2025 Volume 16 - 2025 Edited by Venkata Ramireddy Narala, Yogi Vemana University, India Reviewed by Yssel Mendoza-Mari, Western University of Health Sciences, United States Vira Zlatkina, V