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The rats in the BPC-157 group were randomly subdivided and received doses of 10, 20, or 40 g/kg BPC-157 (n = 7 in each group)
Compromised MPS1 activity induces multipolar spindle formation in oocytes from aged mares: establishing the horse as a natural animal model to study age-induced oocyte meiotic spindle instability
For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)
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