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In recent years there is a drastic increase in drug and alcohol consumption which is a disturbing factor for the welfare of the society

doi: 10.1074/mcp.M111.012203 159 ZhangQFillmoreTLSchepmoesAAClaussTRWGritsenkoMAMuellerPWet al

The total number of the cells was evaluated based on the following formula: where Q was the total number of sexual lineage cells observed in all disectors, h was the optical disector's height, a/f was the area of the counting frame, P was the total number of observed frames, BA was the setting of the microtome, and t was the mean of the final section thickness [36]