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1 mL extract was mixed with 1 mL acid ninhydrin [1.25 g ninhydrin (HiMedia, Mumbai, India) dissolved in 30 mL glacial acetic acid (HiMedia, Mumbai, India) and 20 mL ortho-phosphoric acid (HiMedia, Mumbai, India)] and 1 mL glacial acetic acid (SRL, Mumbai, India)

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Here are some reasons why morning might be the ideal time: Fasted State : Taking glutathione on an empty stomach allows for better absorption, as there are no competing nutrients