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The following primary antibodies and their dilutions were used: anti-Gstt1 (Abcam, ab175418, 1:200 for mouse and human tissues), anti-TromaIII (DSHB, 1:500, for mouse tissues), anti-Cytokeratin (Dako, M351529-2, 1:500, for human tissues), Cytokeratin19-555 (Abcam, 203444, 1:500, for human tissues), anti-PCNA (PC10) (Santa Cruz, sc-56, 1:200, for mouse tissues), anti-Ki67 (Abcam, 1:200, for human tissues), and anti-Fibronectin (Santa Cruz, 271098, 1:200, for mouse and human tissues)

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15 However, the recent FDA approval of sitagliptin/metformin (Janumet) may make sitagliptin a more reasonable first-line choice