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The design of the original SURMOUNT studies did not explicitly capture reproductive stage, which created an inherent limitation in this post hoc analysis

The gut microbiome is not the whole story, but it may be one important part of it, especially when a plateau happens alongside digestive discomfort, low food variety, or signs of weaker gut resilience

These findings indicate Day 30 of liver organoids as the optimal stage for drug metabolism and toxicological investigations, providing a proteomic framework that enhances their utility of iHLOs as physiologically relevant liver models