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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

doi: 10.1016/j.bbi.2017.02.013 7 CervettoCVenturiniAPassalacquaMGuidolinDGenedaniSFuxeKet al

Think of them as tiny messenger molecules that tell your bodys cells how to respondwhether thats repairing tissue, reducing inflammation, or promoting new blood vessel growth

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