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The results of the COS cycle as well as the implantation and pregnancy rates were compared between groups

Then gels were either stained by Instant Blue (Sigma Aldrich) or immediately transferred to PVDF membrane (BioRad) at 100V for 1h

Immunization schedule BALB/c mice were selected for Pf CSP immunization based on an extensive precedent in CSP vaccine literature, where the H-2K d -restricted CD8 + T-cell epitope NYDNAGTNL is recognized, facilitating hypothesis testing aligned with established immunological methods