established an in vitro rabbit corneal tissue model of PAO exposure (5 or 10 g) over a duration of 3, 5, and 10 minutes, followed by treatment with NAC
The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)
A 2006 Achilles-detachment study by Krivic and colleagues in the same journal applied BPC-157 intraperitoneally once daily at 10 g/kg, 10 ng/kg, or 10 pg/kg for 30 days in rats and reported that tendon-to-bone tissue, which did not heal spontaneously in saline controls, recovered with BPC-157 treatment, with directionally improved load-to-failure, stiffness, Young's modulus, and collagen organization at days 121, including in animals where healing had been impaired by 1 mg/kg 6-methylprednisolone though the abstract does not report numeric effect sizes or p-values
Actinic keratosis improves just two days after a freezing removal treatment
