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Methods We performed targeted quantitative liquid chromatography tandem mass-spectrometry for 20 steroid hormones (corticosteroids, androgens, progestogens, and estrogens) in serum from adult female cluster headache patients, both chronic ( n =28) and episodic ( n =23) consisting of 18 paired samples in bout and remission, obtained from the Danish Cluster Headache Biobank in a prospective case-control study

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